Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Eur J Biochem. 1998 Jun 15;254(3):558-64.

Identification of a human 17p-located cDNA encoding a protein of the Snf2-like helicase family.

Author information

  • 1Laboratoire de Biochimie et Biologie moléculaire, CNRS UPR 41, Recombinaisons Génétiques, Faculté de Médecine, Rennes, France. Florence.Aubry@univ-rennes1.fr

Abstract

Following immunoscreening, we have cloned and sequenced a human cDNA encoding a novel member of the expanding helicase family. The deduced protein, designated hZFH (human zinc-finger helicase), contains the seven domains conserved among the helicase superfamily II and four potential zinc-fingers motifs. In particular, hZFH shows significant similarity to some proteins of the Snf2-like family, known to act as transcriptional regulators for multiples genes. Furthermore, hZFH has 68.5% identity to a human Mi-2 autoantigen to which autoantibodies are produced by a subgroup of patients affected by dermatomyositis. Northern-blot analyses have revealed several hZFH mRNAs with quantitative differences in various human tissues. One alternative splice site of hZFH mRNA was demonstrated and others were predicted. We also report the chromosomal localization of gene hZFH to locus 17p13-17p12 by in situ hybridization. Thus, this novel gene appears as a candidate for several malignant and genetic diseases associated with this region of the genome. The combination of these features suggests that hZFH plays an important role in gene regulation.

PMID:
9688266
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Blackwell Publishing
    Loading ...
    Write to the Help Desk