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Mutat Res. 1998 May 25;400(1-2):45-58.

Yeast ARMs (DNA at-risk motifs) can reveal sources of genome instability.

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  • 1Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, 101 Alexander Dr., P.O. Box 12233, Research Triangle Park, NC 27709, USA. gordenin@niehs.nih.gov

Abstract

The genomes of all organisms contain an abundance of DNA repeats which are at-risk for causing genetic change. We have used the yeast Saccharomyces cerevisiae to investigate various repeat categories in order to understand their potential for causing genomic instability and the role of DNA metabolism factors. Several types of repeats can increase enormously the likelihood of genetic changes such as mutation or recombination when present either in wild type or mutants defective in replication or repair. Specifically, we have investigated inverted repeats, homonucleotide runs, and short distant repeats and the consequences of various DNA metabolism mutants. Because the at-risk motifs (ARMs) that we characterized are sensitive indicators, we have found that they are useful tools to reveal new genetic factors affecting genome stability as well as to distinguish subtle differences between alleles.

Copyright 1998 Elsevier Science B.V. All rights reserved.

PMID:
9685581
[PubMed - indexed for MEDLINE]
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