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Nucleic Acids Res. 1998 Aug 15;26(16):3869-70.

A rapid and sensitive assay for histone acetyl-transferase activity.

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  • 1Laboratoire Oncogénèse, Différenciation et Transduction du Signal, CNRS UPR 9079, IFC-01, 7 rue Guy Moquet, 94801 Villejuif, France.

Abstract

Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.

PMID:
9685509
[PubMed - indexed for MEDLINE]
PMCID:
PMC147752
Free PMC Article
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