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Genes Dev. 1998 Jul 15;12(14):2108-13.

Chromosome methylation patterns during mammalian preimplantation development.

Author information

  • 1U 383 Institut National de la Santé et de la Recherche Médicale (INSERM), Necker-Enfants Malades, 75743 Paris Cedex 15, France.

Abstract

DNA methylation patterns were evaluated during preimplantation mouse development by analyzing the binding of monoclonal antibody to 5-methylcytosine (5-MeC) on metaphase chromosomes. Specific chromosome patterns were observed in each cell stage. A banding pattern predominated in chromosomes at the one-cell stage. Banding was replaced at the two-cell stage by an asymmetrical labeling of the sister chromatids. Then, the proportion of asymmetrical chromosomes decreased by one-half at each cell division until the blastocyst stage, and chromosomes became progressively symmetrical and weakly labeled. Our results indicate that chromosome demethylation is associated with each DNA replication and suggest that a passive mechanism predominates during early development.

PMID:
9679055
[PubMed - indexed for MEDLINE]
PMCID:
PMC317005
Free PMC Article

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