Requirement for the leukocyte-specific adapter protein SLP-76 for normal T cell development

Science. 1998 Jul 17;281(5375):416-9. doi: 10.1126/science.281.5375.416.

Abstract

The leukocyte-specific adapter molecule SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kilodaltons) is rapidly phosphorylated on tyrosine residues after receptor ligation in several hematopoietically derived cell types. Mice made deficient for SLP-76 expression contained no peripheral T cells as a result of an early block in thymopoiesis. Macrophage and natural killer cell compartments were intact in SLP-76-deficient mice, despite SLP-76 expression in these lineages in wild-type mice. Thus, the SLP-76 adapter protein is required for normal thymocyte development and plays a crucial role in translating signals mediated by pre-T cell receptors into distal biochemical events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Gene Targeting
  • Immunoglobulin M / blood
  • Killer Cells, Natural / cytology
  • Leukopoiesis*
  • Lymph Nodes / cytology
  • Lymphocyte Activation
  • Lymphocyte Count
  • Macrophages / cytology
  • Mice
  • Mice, Inbred C57BL
  • Phosphoproteins / genetics
  • Phosphoproteins / physiology*
  • Protein-Tyrosine Kinases / metabolism
  • Receptors, Antigen, T-Cell / metabolism
  • Signal Transduction
  • Spleen / cytology
  • T-Lymphocytes / cytology*
  • Thymus Gland / cytology
  • ZAP-70 Protein-Tyrosine Kinase

Substances

  • Adaptor Proteins, Signal Transducing
  • Immunoglobulin M
  • Phosphoproteins
  • Receptors, Antigen, T-Cell
  • SLP-76 signal Transducing adaptor proteins
  • Protein-Tyrosine Kinases
  • ZAP-70 Protein-Tyrosine Kinase
  • Zap70 protein, mouse