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Microb Drug Resist. 1998 Summer;4(2):85-90.

Molecular cloning and DNA sequencing of the Staphylococcus aureus UDP-N-acetylmuramyl tripeptide synthetase (murE) gene, essential for the optimal expression of methicillin resistance.

Author information

  • 1Molecular Genetics Unit, Instituto de Technologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.

Abstract

The Tn551 insertion in mutant RUSA235 of a highly methicillin resistant Staphylococcus aureus strain results in drastic reduction in the level of methicillin resistance and abnormalities, both in the composition of the peptidoglycan and of the cell wall precursor pool. Cloning and sequencing of the inactivated gene indicates that it is the murE gene of Staphylococcus aureus.

PMID:
9650993
[PubMed - indexed for MEDLINE]
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