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An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast.
Research Institute of Molecular Pathology, Vienna, Austria.
Cohesion between sister chromatids during G2 and M phases depends on the "cohesin" protein Scc1p (Mcd1p). Loss of cohesion at the metaphase to anaphase transition is accompanied by Scc1p's dissociation from chromatids, which depends on proteolysis of Pds1p mediated by a ubiquitin protein ligase called the anaphase promoting complex (APC). We show that destruction of Pds1p is the APC's sole role in triggering Scc1p's dissociation from chromatids and that Pds1p forms a stable complex with a 180 kDa protein called Esp1p, which is essential for the dissociation of Scc1p from sister chromatids and for their separation. We propose that the APC promotes sister separation not by destroying cohesins but instead by liberating the "sister-separating" Esp1 protein from its inhibitor Pds1p.
PMID: 9635435 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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The ELG1 clamp loader plays a role in sister chromatid cohesion.
Parnas O, Zipin-Roitman A, Mazor Y, Liefshitz B, Ben-Aroya S, Kupiec M.
PLoS One. 2009; 4(5):e5497. Epub 2009 May 11.
[PLoS One. 2009]
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Shugoshin prevents cohesin cleavage by PP2A(Cdc55)-dependent inhibition of separase.
Clift D, Bizzari F, Marston AL.
Genes Dev. 2009 Mar 15; 23(6):766-80.
[Genes Dev. 2009]
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A handcuff model for the cohesin complex.
Zhang N, Kuznetsov SG, Sharan SK, Li K, Rao PH, Pati D.
J Cell Biol. 2008 Dec 15; 183(6):1019-31.
[J Cell Biol. 2008]
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