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Cell. 1998 Jun 12;93(6):1043-53.

Geminin, an inhibitor of DNA replication, is degraded during mitosis.

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  • 1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

We describe a novel 25 kDa protein, geminin, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin has a destruction box sequence and is ubiquitinated anaphase-promoting complex (APC) in vitro. In synchronized HeLa cells, geminin is absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). We propose that geminin inhibits DNA replication during S, G2, and M phases and that geminin destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

PMID:
9635433
[PubMed - indexed for MEDLINE]
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