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Mutat Res. 1998 Feb 26;398(1-2):131-41.

Construction of a umuC'-luxAB plasmid for the detection of mutagenic DNA repair via luminescence.

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  • 1School of Biological Sciences, The Flinders University of South Australia, Adelaide, Australia.

Abstract

This paper describes a novel system for the detection of mutagenic DNA repair in Escherichia coli. The DNA damage inducible umuC gene of Escherichia coli has been fused to the luxAB genes from Vibrio harvleyi that encode the enzyme luciferase. Mutagenicity has been assessed semi-quantitatively by the induction of bioluminescence. This system is simple, rapid and equivalent in sensitivity to other currently available test procedures. Its use in the detection of known SOS mutagens MMS, MNNG and UV is described.

PMID:
9626973
[PubMed - indexed for MEDLINE]
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