The immunodominant Brugia malayi paramyosin as a marker of current infection with Wuchereria bancrofti adult worms

Infect Immun. 1998 Jun;66(6):2854-8. doi: 10.1128/IAI.66.6.2854-2858.1998.

Abstract

The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S-transferase fusion protein and used to evaluate humoral responses of adults from a W. bancrofti-endemic area in French Polynesia according to their parasitological status. Immunoglobulin G4 (IgG4) preferentially bound to paramyosin in W. bancrofti-parasitized individuals, in contrast to unparasitized individuals, who harbored neither microfilaria nor Og4C3 adult worm circulating antigen. Reduction of the anti-paramyosin IgG4 titer following combined chemotherapy with diethylcarbamazine and ivermectin was significantly correlated with a reduction in the adult worm burden. This indicates that the presence of paramyosin-reactive IgG4 is associated with the presence of parasites and that reduction can be used as an immunological marker for W. bancrofti clearance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Antibodies, Helminth / blood
  • Antigens, Helminth* / genetics
  • Antigens, Helminth* / immunology
  • Biomarkers
  • Brugia malayi / genetics
  • Brugia malayi / immunology*
  • DNA, Complementary / genetics
  • Diethylcarbamazine / therapeutic use
  • Filariasis / diagnosis*
  • Filariasis / epidemiology
  • Filaricides / therapeutic use
  • Glutathione Transferase / genetics
  • Helminth Proteins / genetics
  • Helminth Proteins / immunology
  • Humans
  • Immunodominant Epitopes
  • Ivermectin / therapeutic use
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Polynesia / epidemiology
  • Recombinant Fusion Proteins / immunology
  • Treatment Outcome
  • Tropomyosin* / genetics
  • Tropomyosin* / immunology
  • Wuchereria bancrofti / isolation & purification*

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Biomarkers
  • DNA, Complementary
  • Filaricides
  • Helminth Proteins
  • Immunodominant Epitopes
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Tropomyosin
  • Ivermectin
  • Glutathione Transferase
  • Diethylcarbamazine

Associated data

  • GENBANK/U77590