Abstract
The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S-transferase fusion protein and used to evaluate humoral responses of adults from a W. bancrofti-endemic area in French Polynesia according to their parasitological status. Immunoglobulin G4 (IgG4) preferentially bound to paramyosin in W. bancrofti-parasitized individuals, in contrast to unparasitized individuals, who harbored neither microfilaria nor Og4C3 adult worm circulating antigen. Reduction of the anti-paramyosin IgG4 titer following combined chemotherapy with diethylcarbamazine and ivermectin was significantly correlated with a reduction in the adult worm burden. This indicates that the presence of paramyosin-reactive IgG4 is associated with the presence of parasites and that reduction can be used as an immunological marker for W. bancrofti clearance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Antibodies, Helminth / blood
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Antigens, Helminth* / genetics
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Antigens, Helminth* / immunology
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Biomarkers
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Brugia malayi / genetics
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Brugia malayi / immunology*
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DNA, Complementary / genetics
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Diethylcarbamazine / therapeutic use
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Filariasis / diagnosis*
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Filariasis / epidemiology
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Filaricides / therapeutic use
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Glutathione Transferase / genetics
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Helminth Proteins / genetics
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Helminth Proteins / immunology
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Humans
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Immunodominant Epitopes
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Ivermectin / therapeutic use
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Molecular Sequence Data
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Polynesia / epidemiology
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Recombinant Fusion Proteins / immunology
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Treatment Outcome
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Tropomyosin* / genetics
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Tropomyosin* / immunology
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Wuchereria bancrofti / isolation & purification*
Substances
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Antibodies, Helminth
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Antigens, Helminth
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Biomarkers
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DNA, Complementary
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Filaricides
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Helminth Proteins
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Immunodominant Epitopes
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Peptide Fragments
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Recombinant Fusion Proteins
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Tropomyosin
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Ivermectin
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Glutathione Transferase
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Diethylcarbamazine