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Biochim Biophys Acta. 1998 Apr 1;1397(1):27-30.

Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus.

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  • 1IFIDESA-ARISTEGUI, Research and Development Department, Bilbao, Spain. im000001@sarenet.es

Abstract

The cDNA encoding an allergen from the dust mite Dermatophagoides pteronyssinus has been cloned and sequenced. The allergen (Der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. The final recovery of recombinant Der p 10 from the culture media after a single purification step was as much as 26 mg/l. The recombinant allergen is reactive to shrimp antitropomyosin IgG antibodies and has a 5.6% frequency of IgE reactivity in sera from mite-allergic patients.

PMID:
9545522
[PubMed - indexed for MEDLINE]
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