Abstract
The effects of lipophilic ions on the intramembrane charge movement and intracellular calcium transient were studied using freshly dissociated skeletal muscle cells from mice fetuses. The lipophilic cations Rhodamine 6G and tetraphenylphosphonium (TPP) immobilized part of the intramembrane charge movement in a dose-dependent manner, and inhibited both calcium transient and contraction evoked by membrane depolarization. In contrast, the lipophilic anion 1-anilinonaphthalene-8-sulfonic acid (ANS) had no effect on intramembrane charge movement. We suggest that the lipophilic cations block the voltage-sensing mechanism for the excitation-contraction (E-C) coupling mechanism.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anilino Naphthalenesulfonates / pharmacology
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Animals
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Calcium / metabolism*
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Cells, Cultured
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Coloring Agents / pharmacology
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Dose-Response Relationship, Drug
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Fluorescent Dyes / pharmacology
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Indicators and Reagents / pharmacology
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Intracellular Membranes / chemistry
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Intracellular Membranes / drug effects*
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Mice
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Muscle Contraction / drug effects
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Muscle, Skeletal / drug effects*
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Muscle, Skeletal / embryology
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Muscle, Skeletal / metabolism
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Neuromuscular Depolarizing Agents / pharmacology*
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Onium Compounds / pharmacology*
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Organophosphorus Compounds / pharmacology*
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Rhodamines / chemistry
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Rhodamines / pharmacology*
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Spectrometry, Fluorescence
Substances
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Anilino Naphthalenesulfonates
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Coloring Agents
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Fluorescent Dyes
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Indicators and Reagents
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Neuromuscular Depolarizing Agents
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Onium Compounds
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Organophosphorus Compounds
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Rhodamines
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rhodamine 6G
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1-anilino-8-naphthalenesulfonate
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Calcium
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tetraphenylphosphonium