Biochemistry. 1998 Apr 7;37(14):4767-72.

Structural evidence for the presence of a secondary calcium binding site in human alpha-lactalbumin.

Source

Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U.K.

Abstract

The high-resolution X-ray crystal structure of human alpha-lactalbumin (at 1.8 A) in the presence of an elevated level of calcium reveals a new secondary calcium binding site, 7.9 A away from the primary calcium binding site known in all alpha-lactalbumin structures so far. The new calcium binding site is different from the zinc and sulfate binding sites [Ren, J., et al. (1993) J. Biol. Chem. 268, 19292-19298] but shares common features with the manganese binding site as described by Gerkin [Gerkin, T. A. (1984) Biochemistry 23, 4688-4697]. The proximity of the manganese and calcium binding region and the location of the functional site on one side of the charged surface of the alpha-lactalbumin molecule suggest that these binding sites might play a role in the formation of the lactose synthase complex.

PMID:: 9537992; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

PDB/1A4V

Grant Support

GM21363/GM/NIGMS NIH HHS/United States

LinkOut - more resources

Full Text Sources

Medical

Dietary Proteins - MedlinePlus Health Information

Molecular Biology Databases

CALCIUM, ELEMENTAL - HSDB

Supplemental Content

Save items

Related citations in PubMed

Alpha-lactalbumin possesses a distinct zinc binding site. [J Biol Chem. 1993]
Alpha-lactalbumin possesses a distinct zinc binding site.
Ren J, Stuart DI, Acharya KR. J Biol Chem. 1993 Sep 15; 268(26):19292-8.
Crystal structures of guinea-pig, goat and bovine alpha-lactalbumin highlight the enhanced conformational flexibility of regions that are significant for its action in lactose synthase. [Structure. 1996]
Crystal structures of guinea-pig, goat and bovine alpha-lactalbumin highlight the enhanced conformational flexibility of regions that are significant for its action in lactose synthase.
Pike AC, Brew K, Acharya KR. Structure. 1996 Jun 15; 4(6):691-703.
Co2+ binding to alpha-lactalbumin. [J Protein Chem. 1994]
Co2+ binding to alpha-lactalbumin.
Permyakov EA, Berliner LJ. J Protein Chem. 1994 Apr; 13(3):277-81.
Review alpha-Lactalbumin: structure and function. [FEBS Lett. 2000]
Review alpha-Lactalbumin: structure and function.
Permyakov EA, Berliner LJ. FEBS Lett. 2000 May 19; 473(3):269-74.
Review Metal-ion binding and the molecular conformational properties of alpha lactalbumin. [Crit Rev Biochem Mol Biol. 1989]
Review Metal-ion binding and the molecular conformational properties of alpha lactalbumin.
Kronman MJ. Crit Rev Biochem Mol Biol. 1989; 24(6):565-667.

See reviews... See all...

Cited by 3 PubMed Central articles

Changes in proteasome structure and function caused by HAMLET in tumor cells. [PLoS One. 2009]
Changes in proteasome structure and function caused by HAMLET in tumor cells.
Gustafsson L, Aits S, Onnerfjord P, Trulsson M, Storm P, Svanborg C. PLoS One. 2009; 4(4):e5229. Epub 2009 Apr 14.
Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscL. [Biophys J. 2007]
Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscL.
van den Bogaart G, Krasnikov V, Poolman B. Biophys J. 2007 Feb 15; 92(4):1233-40. Epub 2006 Dec 1.
Characterization of the unfolded state of bovine alpha-lactalbumin and comparison with unfolded states of homologous proteins. [Protein Sci. 2006]
Characterization of the unfolded state of bovine alpha-lactalbumin and comparison with unfolded states of homologous proteins.
Wirmer J, Berk H, Ugolini R, Redfield C, Schwalbe H. Protein Sci. 2006 Jun; 15(6):1397-407.

Structures reported by this article

Alpha-Lactalbumin

PDB: 1A4V

Source: Homo sapiens

Method: X-Ray Diffraction

Resolution: 1.8 Å

See all 3 structures...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Structure
Three-dimensional structure records in the NCBI Structure database for data reported in the current articles.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Structural evidence for the presence of a secondary calcium binding site in huma...
Structural evidence for the presence of a secondary calcium binding site in human alpha-lactalbumin.
Biochemistry. 1998 Apr 7 ;37(14):4767-72.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: