Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF

Leukemia. 1998 Mar;12(3):382-9. doi: 10.1038/sj.leu.2400951.

Abstract

We report a 20-month-old boy with acute lymphoblastic leukemia with the 11q23 translocation whose blasts markedly increased in peripheral blood after intravenous granulocyte colony-stimulating factor (G-CSF) administration, but disappeared after stopping G-CSF. The in vitro study showed that the leukemic cells separated from this patient expressed G-CSF receptor (G-CSFR) and an addition of G-CSF stimulated their proliferation by 3H-thymidine incorporation assay (stimulation index, 4.9). To clarify whether or not leukemic cells with 11q23 translocations generally express G-CSFR and show proliferative response to G-CSF, we performed the similar in vitro experiments using eight leukemic cell lines with 11q23 translocations. We found that all cell lines examined expressed G-CSFR (20-98%) and proliferation of seven leukemic cell lines was significantly enhanced in response to G-CSF (stimulation index >1.5 in five cell lines), suggesting a possible participation of the G-CSF/G-CSFR interaction in the process of growth regulation of leukemic cells with 11q23 translocations.

Publication types

  • Case Reports

MeSH terms

  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / pathology
  • Blast Crisis
  • Cell Division / drug effects
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Cytarabine / administration & dosage
  • DNA Replication / drug effects
  • Etoposide / administration & dosage
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Granulocyte Colony-Stimulating Factor / therapeutic use*
  • Granulocytes / drug effects
  • Granulocytes / pathology
  • Humans
  • Immunophenotyping
  • Infant
  • Male
  • Mitoxantrone / administration & dosage
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / blood
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / therapy
  • Receptors, Granulocyte Colony-Stimulating Factor / biosynthesis*
  • Receptors, Granulocyte Colony-Stimulating Factor / blood
  • Tumor Cells, Cultured

Substances

  • Receptors, Granulocyte Colony-Stimulating Factor
  • Cytarabine
  • Granulocyte Colony-Stimulating Factor
  • Etoposide
  • Mitoxantrone