Antibody to a Helicobacter pylori species specific antigen in patients with adenocarcinoma of the stomach

Biochem Biophys Res Commun. 1998 Mar 17;244(2):360-3. doi: 10.1006/bbrc.1998.8271.

Abstract

This study attempted to identify a possible antibody response to Helicobacter pylori, which is associated with patients with adeno-carcinoma of the stomach. By using proteins of H. pylori as the antigen, pooled sera from gastric cancer and non-cancer patients were used as the first antibody for Western blot analysis. Antibody responses to a 26 kD secreted protein were observed in pooled cancer sera, but not in pooled sera from non-cancer patients. The protein was purified, while amino acid sequences revealed that it was a H. pylori species specific protein. The gene of this protein was cloned and a recombinant protein was expressed in E. coli. In addition, an antibody to the recombinant protein was tested in each individual patient using Western blot analysis. None of the forty non-gastric cancer patients were positive for the antibody to the recombinantly expressed 26 kD species specific protein. Meanwhile, six of the twenty four cancer patients tested positive (0/40 vs 6/24, p < 0.01). Results presented herein demonstrate that the species specific protein of H. pylori can be useful in detecting H. pylori associated with adenocarcinoma of the stomach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / etiology
  • Adenocarcinoma / immunology*
  • Adenocarcinoma / microbiology*
  • Amino Acid Sequence
  • Antibodies, Bacterial*
  • Antigens, Bacterial* / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Genes, Bacterial
  • Helicobacter Infections / complications
  • Helicobacter Infections / immunology
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / genetics
  • Helicobacter pylori / immunology*
  • Helicobacter pylori / pathogenicity
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Stomach Neoplasms / etiology
  • Stomach Neoplasms / immunology*
  • Stomach Neoplasms / microbiology*
  • Virulence

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • DNA Primers
  • DNA, Bacterial
  • Recombinant Proteins