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Anal Biochem. 1998 Mar 1;257(1):80-8.

Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography.

Author information

  • 1Department of Genetics, Flanders Institute for Biotechnology (VIB), Universiteit Gent, Belgium.

Abstract

The pyrrolophenanthridine alkaloid lycorine has frequently been used as a specific inhibitor to help elucidate the function of ascorbic acid (vitamin C) in a wide range of biological processes. It was recently reported that this function can be exercised by inhibiting the activity of L-galactono-1,4-lactone dehydrogenase, the terminal enzyme of ascorbic acid biosynthesis, although working with the purified enzyme, we have been unable to repeat this result. Here, we present a procedure for the purification and analysis of lycorine by high-performance liquid chromatography from two Crinum species and describe for the first time a method that allows the simultaneous analysis of ascorbic acid and lycorine in tissue extracts of Crinum asiaticum by micellar electrokinetic chromatography.

PMID:
9512776
[PubMed - indexed for MEDLINE]
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