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J Virol Methods. 1997 Dec;69(1-2):197-208.

Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay.

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  • 1Scitech Genetics Ltd., The Maxwell, Singapore, Singapore.

Abstract

A rapid assay for the quantification of hepatitis B virus DNA in human serum was developed. The principle of the method combines competitive polymerase chain reaction (cPCR) (for the controlled amplification of hepatitis B virus DNA) and scintillation proximity assay (SPA) technology (for rapid detection and quantitation of PCR products). It also incorporates a reproducible and simple method for the preparation of serum DNA suitable for PCR amplification. The assay has a better linear dynamic range than traditional methods that use 32P to detect PCR products. It was applied to a range of hepatitis B virus (HBV) surface antigen positive (HBsAg + ) sera, and shown to be more sensitive than a commercially available HBV DNA kit.

PMID:
9504765
[PubMed - indexed for MEDLINE]
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