Abstract
We have cloned a new gene, pdh1, from genomic DNA of fission yeast Schizosaccharomyces pombe. pdh1 is actively transcribed as 1400-nucleotide mRNA in vegetatively growing cells and can code for a 226 amino acid polypeptide (pdh1p). Computational structural prediction has revealed that the pdh1p is a highly hydrophobic protein with seven transmembrane domains. The prediction has also detected a possible C-kinase phosphorylation site within the longest hydrophilic loop.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Cell Membrane / metabolism
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DNA, Plant
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Deoxyribonuclease HindIII / metabolism
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Fungal Proteins / genetics*
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / growth & development
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Schizosaccharomyces pombe Proteins*
Substances
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DNA, Plant
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Fungal Proteins
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Membrane Proteins
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Schizosaccharomyces pombe Proteins
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pdh1 protein, S pombe
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Deoxyribonuclease HindIII
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Deoxyribonucleases, Type II Site-Specific
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GGTACC-specific type II deoxyribonucleases