Abstract
The rat hippocalcin gene -3.2 to +0.6 kb region activates reporter gene expression in the NG108-15 and PC12 neuronal cell lines, but not in NIH3T3 or HEK-293 cells. Three fragments (-3.2 to -2.6, -2.6 to -2.3 and -2.3 to -1.8 kb) weakly activate transcription, and "-1.8 to -1.5" kb is a strong activator. Thus cell type-specific expression of the rat hippocalcin gene is regulated by distributed elements in the -3.2 to -1.5 kb region.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Calcium-Binding Proteins / biosynthesis*
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Calcium-Binding Proteins / genetics*
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Cell Line
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Chloramphenicol O-Acetyltransferase / biosynthesis
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DNA / genetics*
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DNA, Neoplasm / genetics
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Glioma
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Hippocalcin
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Humans
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins*
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Neuroblastoma
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Neurons / metabolism
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PC12 Cells
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Rats
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Recombinant Fusion Proteins / biosynthesis
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Transcription, Genetic*
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Transfection
Substances
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Calcium-Binding Proteins
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DNA, Neoplasm
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HPCA protein, human
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Hpca protein, mouse
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Hpca protein, rat
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Nerve Tissue Proteins
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Recombinant Fusion Proteins
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Hippocalcin
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DNA
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Chloramphenicol O-Acetyltransferase
Associated data
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GENBANK/X96993
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GENBANK/Y12514