DNA regions supporting hippocalcin gene expression in cell lines

Brain Res Mol Brain Res. 1997 Dec 15;52(2):323-5. doi: 10.1016/s0169-328x(97)00293-3.

Abstract

The rat hippocalcin gene -3.2 to +0.6 kb region activates reporter gene expression in the NG108-15 and PC12 neuronal cell lines, but not in NIH3T3 or HEK-293 cells. Three fragments (-3.2 to -2.6, -2.6 to -2.3 and -2.3 to -1.8 kb) weakly activate transcription, and "-1.8 to -1.5" kb is a strong activator. Thus cell type-specific expression of the rat hippocalcin gene is regulated by distributed elements in the -3.2 to -1.5 kb region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Calcium-Binding Proteins / biosynthesis*
  • Calcium-Binding Proteins / genetics*
  • Cell Line
  • Chloramphenicol O-Acetyltransferase / biosynthesis
  • DNA / genetics*
  • DNA, Neoplasm / genetics
  • Glioma
  • Hippocalcin
  • Humans
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Neuroblastoma
  • Neurons / metabolism
  • PC12 Cells
  • Rats
  • Recombinant Fusion Proteins / biosynthesis
  • Transcription, Genetic*
  • Transfection

Substances

  • Calcium-Binding Proteins
  • DNA, Neoplasm
  • HPCA protein, human
  • Hpca protein, mouse
  • Hpca protein, rat
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • Hippocalcin
  • DNA
  • Chloramphenicol O-Acetyltransferase

Associated data

  • GENBANK/X96993
  • GENBANK/Y12514