Appl Microbiol Biotechnol. 1998 Jan;49(1):31-8.

Purification and characterization of recombinant spider silk expressed in Escherichia coli.

Arcidiacono S, Mello C, Kaplan D, Cheley S, Bayley H.

Source

Biotechnology Division, U.S. Army Natick Army Research, Development, and Engineering Center, Natick, MA 01760, USA.

Abstract

A partial cDNA clone, from the 3' end of the dragline silk gene was isolated from Nephila clavipes major ampullate glands. This clone contains a 1.7-kb insert, consisting of a repetitive coding region of 1.4-kb and a 0.3-kb nonrepetitive coding region; 1.5-kb of the 1.7-kb fragment was cloned into Escherichia coli and a 43-kDa recombinant silk protein was expressed. Characterization of the purified protein by Western blot, amino acid composition analysis, and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry confirms it to be spider dragline silk.

PMID:: 9487707; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances, Secondary Source ID

MeSH Terms

Substances

Secondary Source ID

GENBANK/U20329

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Construction, cloning, and expression of synthetic genes encoding spider dragline silk. [Biochemistry. 1995]
Construction, cloning, and expression of synthetic genes encoding spider dragline silk.
Prince JT, McGrath KP, DiGirolamo CM, Kaplan DL. Biochemistry. 1995 Aug 29; 34(34):10879-85.
[Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli]. [Sheng Wu Gong Cheng Xue Bao. 2...]
[Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli].
Li M, Zhang WX, Huang ZH, Huang JK. Sheng Wu Gong Cheng Xue Bao. 2002 May; 18(3):331-4.
[Cloning and prokaryotic expression of major ampullate spidroin gene of spider]. [Sheng Wu Gong Cheng Xue Bao. 2...]
[Cloning and prokaryotic expression of major ampullate spidroin gene of spider].
Pan HC, Song DX, Zhou KY, Zhu GP. Sheng Wu Gong Cheng Xue Bao. 2007 May; 23(3):446-51.
Review Microbial production of spider silk proteins. [J Biotechnol. 2000]
Review Microbial production of spider silk proteins.
Fahnestock SR, Yao Z, Bedzyk LA. J Biotechnol. 2000 Aug; 74(2):105-19.
Review The mechanical design of spider silks: from fibroin sequence to mechanical function. [J Exp Biol. 1999]
Review The mechanical design of spider silks: from fibroin sequence to mechanical function.
Gosline JM, Guerette PA, Ortlepp CS, Savage KN. J Exp Biol. 1999 Dec; 202(Pt 23):3295-303.

See reviews... See all...

Cited by 12 PubMed Central articles

Natural and Genetically Engineered Proteins for Tissue Engineering. [Prog Polym Sci. 2012]
Natural and Genetically Engineered Proteins for Tissue Engineering.
Gomes S, Leonor IB, Mano JF, Reis RL, Kaplan DL. Prog Polym Sci. 2012 Jan 1; 37(1):1-17.
Review Multivalent protein polymers with controlled chemical and physical properties. [Adv Drug Deliv Rev. 2010]
Review Multivalent protein polymers with controlled chemical and physical properties.
Top A, Kiick KL. Adv Drug Deliv Rev. 2010 Dec 30; 62(15):1530-40. Epub 2010 Jun 1.
Antheraea pernyi silk fiber: a potential resource for artificially biospinning spider dragline silk. [J Biomed Biotechnol. 2010]
Antheraea pernyi silk fiber: a potential resource for artificially biospinning spider dragline silk.
Zhang Y, Yang H, Shao H, Hu X. J Biomed Biotechnol. 2010; 2010:683962. Epub 2010 May 5.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Purification and characterization of recombinant spider silk expressed in Escher...
Purification and characterization of recombinant spider silk expressed in Escherichia coli.
Appl Microbiol Biotechnol. 1998 Jan ;49(1):31-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: