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Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2073-6.

Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA.

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  • 1Center for Biomolecular Recognition, Departments of Medical Biochemistry and Genetics, Biochemistry B, The Panum Institute, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Copenhagen, Denmark.

Abstract

Peptide nucleic acid (PNA) is a DNA mimic that has shown considerable promise as a lead compound for developing gene therapeutic drugs. We report that PNAs targeted to functional and accessible sites in ribosomal RNA can inhibit translation in an Escherichia coli cell-free transcription/translation system, with 50% reductions caused by nanomolar PNA concentrations. The effect in vitro is quantitatively similar to that of the known translation inhibitor and antibiotic tetracycline. Also, the targeted PNAs inhibited bacterial growth on agar plates and in liquid culture. A strain of E. coli (AS19) that is more permeable to antibiotics was approximately 10-fold more sensitive to the active PNAs, suggesting that the effect on growth indeed was caused by PNAs that entered cells. Inhibition was not observed when using control PNAs of similar composition but with an unrelated or mismatched sequence. The results demonstrate that ribosomal RNA is a possible target for sequence-designed novel antibiotics based on DNA analogues or mimics.

PMID:
9482840
PMCID:
PMC19253
[PubMed - indexed for MEDLINE]
Free PMC Article
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