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Parasitology. 1998 Jan;116 ( Pt 1):43-50.

Re-examination of resistance of Toxoplasma gondii tachyzoites and bradyzoites to pepsin and trypsin digestion.

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  • United States Department of Agriculture, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705-2350, USA. JDUBEY@GGPL.ARSUSDA.GOV

Abstract

The effect of digestion in trypsin and acid pepsin on Toxoplasma gondii tachyzoites and bradyzoites was re-evaluated because of recent use of this method to distinguish tachyzoites from bradyzoites. Toxoplasma gondii tachyzoites survived better in 0.5% trypsin solution for 1 h than in 1.0% solution, and occasionally survived for 2 h in acid pepsin solution. Extracellular tachyzoites (> or = 1000) were also infectious orally to mice and cats. Bradyzoites survived equally in trypsin and acid pepsin solutions but the digestion of brain tissue in 0.5% trypsin solution was better than in acid pepsin solution. The resistance to digestion in acid pepsin solution is not a reliable method to distinguish tachyzoites from bradyzoites.

PMID:
9481773
[PubMed - indexed for MEDLINE]
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