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Genome Res. 1998 Feb;8(2):135-45.

A physical map of the Leishmania major Friedlin genome.

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  • 1Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK. a.ivens@ic.ac.uk


An extensive physical map of the Leishmania major Friedlin genome has been assembled by the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization. The integrated data obtained for 9004 fingerprinted clones and 974 probes have placed 91.2% of the 33.58-Mb genome into contigs representing each of the 36 chromosomes. This first-generation map has already provided a suitable framework for both high-throughput DNA sequencing and functional studies of the L. major parasite.

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