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Nat Genet. 1998 Feb;18(2):136-41.

An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination.

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  • 1Department of Anatomy, University of California, San Francisco 94143-0452, USA.

Abstract

We describe a strategy for generating an allelic series of mutations at a given locus that requires the production of only one targetted mouse line. The 'allelogenic' mouse line we produced carries a hypomorphic allele of Fgf8, which can be converted to a null allele by mating to cre transgenic animals. The hypomorphic allele can also be reverted to wild-type by mating the allelogenic mice to flp transgenic animals, thereby generating a mouse line suitable for Cre-induced tissue-specific knockout experiments. Analysis of embryos carrying different combinations of these alleles revealed requirements for Fgf8 gene function during gastrulation, as well as cardiac, craniofacial, forebrain, midbrain and cerebellar development.

PMID:
9462741
[PubMed - indexed for MEDLINE]
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