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J Bacteriol. 1998 Feb;180(3):746-8.

A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake.

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  • 1Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA. mlgwinn@tigr.org


Haemophilus influenzae Rd is a gram-negative natural transformer. A mutant strain, RJ248, that has normal DNA uptake and translocation but whose transformation frequency is 300 times lower than that of wild-type H. influenzae and whose phage recombination is 8 times lower was isolated. The affected gene, comM, is induced during competence development in wild-type H. influenzae but not in RJ248.

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