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1: Mol Biotechnol. 1997 Dec;8(3):279-81.Links

Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose.

Bøg-Hansen TC.

Protein Laboratory, Panum Institute, University of Copenhagen, Denmark. tcbh@biobase.dk

A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbent is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatographic procedure is simply adsorption, wash, elution. The procedure is simple, efficient, and inexpensive.

PMID: 9438262 [PubMed - indexed for MEDLINE]