Abstract

The neu/cerb-B2 gene is frequently amplified and/or overexpressed in human epithelial ovarian cancers. We have established an inbred animal model for ovarian cancer that mimics aspects of human ovarian cancer by transducing a spontaneously immortalized rat ovarian surface epithelial cell line in culture with ecotropic retroviruses expressing a mutated rat neu/c-erb-B2 oncogene. Transfectants expressing neu at a high level exhibited altered morphology and behavior in two-dimensional and three-dimensional culture in Matrigel, could be cloned in soft agar, and were more invasive through a Matrigel membrane than control transfectants transduced with a similar retrovirus expressing the beta-galactosidase gene. When injected intraperitoneally, neu-expressing transfectants produced highly invasive, rapidly growing tumors that coated the peritoneal cavity and induced ascites formation. Furthermore, neu transfectants could be grown as solid tumors when injected subepithelially into the ovary. The neu-transfected cells also formed tumors when injected subcutaneously into the mammary fat pad, although they grew relatively poorly and often regressed. Transfectants expressing beta-galactosidase failed to produce tumors at any of the sites injected. A second rat ovarian surface epithelial cell line was similarly transduced with the neu/c-erb-B2-expressing retrovirus. However, transformed phenotypes and tumorigenicity were not induced in this cell line. These experiments show directly that overexpression of neu in an established line of rat ovarian epithelium is extremely oncogenic. This animal model system may prove useful for the study of ovarian cancer biology in immunocompetent animals.