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Science. 1997 Dec 19;278(5346):2123-6.

Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex.

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  • 1Howard Hughes Medical Institute, Laboratory of Cell Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. beckmar@rockvax.rockefeller.edu

Abstract

An oligomer of the Sec61 trimeric complex is thought to form the protein-conducting channel for protein transport across the endoplasmic reticulum. A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion. Cryo-electron microscopy of the ribosome-Sec61 complex and a three-dimensional reconstruction showed that the Sec61 oligomer is attached to the large ribosomal subunit by a single connection. Moreover, a funnel-shaped pore in the Sec61 oligomer aligned with the exit of a tunnel traversing the large ribosomal subunit, strongly suggesting that both structures function together in the translocation of proteins across the endoplasmic reticulum membrane.

Comment in

  • Tinkering with nature. [Nat Rev Mol Cell Biol. 2011]
  • A ribosome at the end of the tunnel. [Science. 1997]
PMID:
9405348
[PubMed - indexed for MEDLINE]
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