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FEBS Lett. 1997 Nov 10;417(2):177-83.

Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases.

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  • 1CNRS ERS 575, Institut de Biologie Physico-Chimique, Paris, France.

Abstract

The unc-47 locus of Caenorhabditis elegans has been suggested to encode a synaptic vesicle GABA transporter. Here we used hydropathy plot analysis to identify a candidate vesicular GABA transporter in genomic sequences derived from a region of the physical map comprising unc-47. A mouse homologue was identified and cloned from EST database information. In situ hybridization in rat brain revealed codistribution with both GABAergic and glycinergic neuronal markers. Moreover, expression in COS-7 and PC12 cells induced an intracellular, glycine-sensitive GABA uptake activity. These observations, consistent with previous data on GABA and glycine uptake by synaptic vesicles, demonstrate that the mouse clone encodes a vesicular inhibitory amino acid transporter.

PMID:
9395291
[PubMed - indexed for MEDLINE]
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