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Infect Immun. 1997 Dec;65(12):5376-80.

Quantification of conserved antigens in Helicobacter pylori during different culture conditions.

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  • 1Department of Medical Microbiology and Immunology, Göteborg University, Sweden.

Abstract

In this study, we raised monoclonal antibodies (MAbs) against three conserved Helicobacter pylori antigens, i.e., the N-acetylneuraminyllactose-binding fibrillar hemagglutinin, HpaA; the flagellin subunits, FlaA and FlaB; and a species-specific 26-kDa protein. The MAbs were used for the development of sensitive inhibition enzyme-linked immunosorbent assays for quantification of these antigens in H. pylori during various culture conditions. The quantities of these antigens varied considerably (up to 8-fold) during different culture procedures and between strains (up to 10-fold).

PMID:
9393845
PMCID:
PMC175778
[PubMed - indexed for MEDLINE]
Free PMC Article
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