Antitumor-I (AT-I) is known as an effective traditional Chinese medicine to be used in combination with chemotherapeutic agents against tumor. It was demonstrated that AT-I could inhibit tumor growth and modulate IL-2, IL-6 and NK cell activities. In order to improve its preparation, we got the alcohol extract from AT-I (AEA-I) by using natural pharmaceutical alcohol extract method, and compared the effect of AEA-I on cytokins with that of AT-I. The results show that AEA-I has a good positive correlation with AT-I on IL-2 and IL-6 activities of mice in vivo.