The experiments were performed on the anesthetized rats. Single unit was recorded extracellularlly from the thalamic nucleus submedius (Sm) with glass micropipettes. The responses of Sm neurons were examined to electrical stimulation of the peroneal nerve and "Zusanli" point. The results show that most (81%) of the neurons in Sm responded to electrical stimulation of the peroneal nerve with afferent C fiber excitation, and this response increased following increase of the stimulation intensity and pulse numbers. It was found that electrical stimulation of the "Zusanli" point could activate the Sm neuronal activities with higher intensity threshold compared to that of the peroneal nerve stimulation, and the response properties of Sm neurons to acupoint stimuli were similar to those to stimulation of peroneal nerve. These results provide support for the hypothesis that a negative feedback loop consisting of spinal cord-Sm-VLO-PAG-spinal cord responsible for nociceptive modulation is involved in acupuncture analgesia, especially in analgesia produced by electroacupuncture-evoked activation of the afferent C fibers.