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Nucleic Acids Res. 1997 Oct 1;25(19):3957-8.

Betaine improves the PCR amplification of GC-rich DNA sequences.

Author information

  • 1Research Division of the Department of Urology, University Hospital Charité, Humboldt University, D-10098 Berlin, Germany. henke@rz.charite.hu-berlin.de

Abstract

Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.

PMID:
9380524
[PubMed - indexed for MEDLINE]
PMCID:
PMC146979
Free PMC Article
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