Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biochem J. 1997 Dec 1;328 ( Pt 2):409-14.

Reconstitution of native-like nucleosome core particles from reversed-phase-HPLC-fractionated histones.

Author information

  • 1Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada V8W 3P6.

Abstract

We have reconstituted nucleosome core particles from reversed-phase-HPLC-purified chicken erythrocyte core histones and 145 bp random-sequence DNA fragments. Characterization of the resulting nucleoprotein complexes by sedimentation velocity, CD and DNase I footprinting showed that they are structurally indistinguishable from native nucleosome core particles. Furthermore, we have shown that the ability to reproduce these native-like structural features in these reconstituted nucleosome core particles is basically independent of the biological source or the method used (i.e. salt versus acid) for the extraction of histones before their HPLC fractionation. The usefulness and relevance of this approach for the reconstitution of native-like chromatin structures from histone types (histone variants/post-translationally modified histones), which are usually available only in relatively small amounts, is discussed.

PMID:
9371695
[PubMed - indexed for MEDLINE]
PMCID:
PMC1218935
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Portland Press Icon for PubMed Central
    Loading ...
    Write to the Help Desk