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J Cell Biol. 1997 Nov 17;139(4):895-905.

Functional expression cloning and characterization of SFT, a stimulator of Fe transport.

Author information

  • 1Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115, USA.

Erratum in

  • J Cell Biol 1999 Oct 4;147(1):following 204.

Abstract

A stimulator of Fe transport (SFT) was identified by functional expression cloning in Xenopus oocytes. SFT-mediated transport has properties defined for transferrin-independent Fe uptake, but its cytolocalization in recycling endosomes and the observed stimulation of transferrin-bound Fe assimilation indicate a key role in intracellular Fe membrane transport as well. SFT has six predicted transmembranous domains and a functionally important RExxE motif that resembles domains involved in yeast Fe transport and Fe-binding by ferritin L-chains. The observation that SFT oligomerizes, along with other structural and mechanistic features, suggests it may be a member of either the ATP-binding cassette or cation diffusion facilitator families. The 3' untranslated region of SFT contains a translation inhibitory element and inhibition of SFT expression in Xenopus oocytes was found to be relieved by coinjection of transcripts from other defined cDNAs that are also described in this report. SFT is the first component of the mammalian Fe membrane transport machinery to be identified.

PMID:
9362508
[PubMed - indexed for MEDLINE]
PMCID:
PMC2139974
Free PMC Article

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