Abstract
Recent findings have suggested that the vertebrate trp family of channel proteins is the structural basis for Ca2+ influx through the capacitative calcium entry (CCE) pathway. We have discerned, in bovine aortic endothelial cells, the concomitant expression of four such vertebrate genes: trp-1 (two splice variants), trp-3, trp-4 and trp-5. Exogenous hormones rendered dynamic effects on the transcript levels of these genes. Most notably, beta-estradiol significantly down-regulated trp-4 while trans-retinoic acid dramatically up-regulated trp-5; yet these hormones rendered little change in CCE. These findings suggest that the extent of a given trp channel's participation in CCE is not reflected in alterations of its transcript level.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Aorta
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Blotting, Southern
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Calcium / metabolism
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Calcium Channels / biosynthesis
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Calcium Channels / genetics*
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Cattle
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Cells, Cultured
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DNA Primers
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism*
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Estradiol / pharmacology
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Gene Expression Regulation / drug effects*
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Hormones / pharmacology*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, DNA
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TRPC Cation Channels
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Tretinoin / pharmacology
Substances
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Calcium Channels
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DNA Primers
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Hormones
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TRPC Cation Channels
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transient receptor potential cation channel, subfamily C, member 1
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Estradiol
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Tretinoin
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Calcium
Associated data
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GENBANK/AF012900
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GENBANK/AF012901