Concomitant and hormonally regulated expression of trp genes in bovine aortic endothelial cells

FEBS Lett. 1997 Oct 6;415(3):335-40. doi: 10.1016/s0014-5793(97)01155-1.

Abstract

Recent findings have suggested that the vertebrate trp family of channel proteins is the structural basis for Ca2+ influx through the capacitative calcium entry (CCE) pathway. We have discerned, in bovine aortic endothelial cells, the concomitant expression of four such vertebrate genes: trp-1 (two splice variants), trp-3, trp-4 and trp-5. Exogenous hormones rendered dynamic effects on the transcript levels of these genes. Most notably, beta-estradiol significantly down-regulated trp-4 while trans-retinoic acid dramatically up-regulated trp-5; yet these hormones rendered little change in CCE. These findings suggest that the extent of a given trp channel's participation in CCE is not reflected in alterations of its transcript level.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aorta
  • Blotting, Southern
  • Calcium / metabolism
  • Calcium Channels / biosynthesis
  • Calcium Channels / genetics*
  • Cattle
  • Cells, Cultured
  • DNA Primers
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Estradiol / pharmacology
  • Gene Expression Regulation / drug effects*
  • Hormones / pharmacology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • TRPC Cation Channels
  • Tretinoin / pharmacology

Substances

  • Calcium Channels
  • DNA Primers
  • Hormones
  • TRPC Cation Channels
  • transient receptor potential cation channel, subfamily C, member 1
  • Estradiol
  • Tretinoin
  • Calcium

Associated data

  • GENBANK/AF012900
  • GENBANK/AF012901