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Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12297-302.

RNA-peptide fusions for the in vitro selection of peptides and proteins.

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  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

Abstract

Covalent fusions between an mRNA and the peptide or protein that it encodes can be generated by in vitro translation of synthetic mRNAs that carry puromycin, a peptidyl acceptor antibiotic, at their 3' end. The stable linkage between the informational (nucleic acid) and functional (peptide) domains of the resulting joint molecules allows a specific mRNA to be enriched from a complex mixture of mRNAs based on the properties of its encoded peptide. Fusions between a synthetic mRNA and its encoded myc epitope peptide have been enriched from a pool of random sequence mRNA-peptide fusions by immunoprecipitation. Covalent RNA-peptide fusions should provide an additional route to the in vitro selection and directed evolution of proteins.

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PMID:
9356443
[PubMed - indexed for MEDLINE]
PMCID:
PMC24913
Free PMC Article

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