J Clin Microbiol. 1997 Nov;35(11):2977-80.

Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day.

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Washington State Department of Health, Public Health Laboratories, Seattle 98155, USA.

Abstract

Genomic DNA patterns generated by pulsed-field gel electrophoresis are highly specific for different strains of an organism and have significant value in epidemiologic investigations of infectious-disease outbreaks. Unfortunately, time-consuming and tedious specimen processing is an inherent problem which limits the use of this powerful technology as a real-time epidemic investigational tool. Here, I describe a rapid method to improve the response time and provide specific bacterial strain identification for the typing of Escherichia coli O157:H7 and other gram-negative organisms in a single day.

PMID:: 9350772; [PubMed - indexed for MEDLINE]
PMCID:: PMC230100

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MeSH Terms

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DNA, Bacterial

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