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Biochem Mol Biol Int. 1997 Oct;43(2):263-8.

Effect of 4-methylumbelliferone on cell-free synthesis of hyaluronic acid.

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  • 1Department of Biochemistry, Hirosaki University School of Medicine, Japan.

Abstract

Human skin fibroblasts were cultured in the presence of 0.5 mM 4-methylumbelliferone for 12 h, and cell-free synthesis of hyaluronic acid was performed using membrane-rich fraction from the cells. The preincubation of the cells with 4-methylumbelliferone reduced hyaluronic acid synthesis to 15% of that of non-preincubated cells, although its chain length was not changed. On the other hand, without preincubation of the cells with 4-methylumbelliferone, hyaluronic acid synthesis was not changed even when 4-methylumbelliferone was added directly to the reaction mixture. These results suggest that 4-methylumbelliferone represses the expression of hyaluronic acid synthase on the cell surface.

PMID:
9350333
[PubMed - indexed for MEDLINE]
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