Abstract
An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25 pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / isolation & purification*
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Antiviral Agents / pharmacology
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Chromatography, Ion Exchange
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Electrophoresis, Polyacrylamide Gel
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Isoelectric Focusing
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Molecular Weight
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N-Glycosyl Hydrolases*
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Plant Leaves / chemistry
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Plant Proteins / isolation & purification*
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Plant Proteins / pharmacology
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Plants, Medicinal / chemistry*
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Rabbits
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Reticulocytes / enzymology
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Ribosome Inactivating Proteins, Type 1
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Tobacco Mosaic Virus / drug effects
Substances
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Antiviral Agents
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Plant Proteins
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Ribosome Inactivating Proteins, Type 1
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N-Glycosyl Hydrolases
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pokeweed antiviral protein