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    Gene. 1997 Sep 1;196(1-2):19-23.

    Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression.

    Sun B, Jeyaseelan K, Chung MC, Teo TS.

    Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore.

    A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of approx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional.

    PMID: 9322736 [PubMed - indexed for MEDLINE]

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