Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell Biol. 1997 Oct;17(10):5996-6001.

In vivo regulation of alternative pre-mRNA splicing by the Clk1 protein kinase.

Author information

  • 1Ottawa Regional Cancer Centre, Ontario, Canada.

Abstract

Controlled expression of cellular and viral genes through alternative precursor messenger RNA (pre-mRNA) splicing requires serine/arginine-rich (SR) proteins. The Clk1 kinase, which phosphorylates SR proteins, is regulated through alternative splicing of the Clk1 pre-mRNA, yielding mRNAs encoding catalytically active and truncated inactive polypeptides (Clk1 and Clk1T, respectively). We present evidence that Clk1 and Clk1T proteins regulate the splicing of Clk1 and adenovirus pre-mRNAs in vivo. The peptide domain encoded by the alternatively spliced exon of Clk1 is essential for the regulatory activity of the Clk1 kinase. This is the first direct demonstration of an in vivo link between alternative splicing and protein kinase activity.

PMID:
9315658
[PubMed - indexed for MEDLINE]
PMCID:
PMC232448
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk