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Science. 1997 Oct 3;278(5335):141-4.

Evidence for a role of CRM1 in signal-mediated nuclear protein export.

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  • 1Institut Curie-CNRS Unité Mixte de Recherche 144, 26 rue d'Ulm, 75248 Paris Cedex 05, France.


Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (NES). This interaction was inhibited by leptomycin B, a drug that prevents the function of the CRM1 protein in yeast. To analyze the role of the CRM1-NES interaction in nuclear export, a transport assay based on semipermeabilized cells was developed. In this system, which reconstituted NES-, cytosol-, and energy-dependent nuclear export, leptomycin B specifically blocked export of NES-containing proteins. Thus, the CRM1 protein could act as a NES receptor involved in nuclear protein export.

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