NF-AT activation induced by a CAML-interacting member of the tumor necrosis factor receptor superfamily

Science. 1997 Oct 3;278(5335):138-41. doi: 10.1126/science.278.5335.138.

Abstract

Activation of the nuclear factor of activated T cells transcription factor (NF-AT) is a key event underlying lymphocyte action. The CAML (calcium-modulator and cyclophilin ligand) protein is a coinducer of NF-AT activation when overexpressed in Jurkat T cells. A member of the tumor necrosis factor receptor superfamily was isolated by virtue of its affinity for CAML. Cross-linking of this lymphocyte-specific protein, designated TACI (transmembrane activator and CAML-interactor), on the surface of transfected Jurkat cells with TACI-specific antibodies led to activation of the transcription factors NF-AT, AP-1, and NFkappaB. TACI-induced activation of NF-AT was specifically blocked by a dominant-negative CAML mutant, thus implicating CAML as a signaling intermediate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Calcineurin
  • Calmodulin-Binding Proteins / metabolism
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Membrane / metabolism
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Jurkat Cells
  • Lymphocyte Activation
  • Membrane Proteins*
  • Molecular Sequence Data
  • Mutation
  • NF-kappa B / metabolism
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Phosphoprotein Phosphatases / metabolism
  • Receptors, Tumor Necrosis Factor / chemistry
  • Receptors, Tumor Necrosis Factor / genetics
  • Receptors, Tumor Necrosis Factor / metabolism*
  • Sequence Alignment
  • Signal Transduction
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • Transcription Factor AP-1 / metabolism
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transfection
  • Transmembrane Activator and CAML Interactor Protein

Substances

  • Adaptor Proteins, Signal Transducing
  • CAMLG protein, human
  • Calmodulin-Binding Proteins
  • Carrier Proteins
  • DNA-Binding Proteins
  • Membrane Proteins
  • NF-kappa B
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Receptors, Tumor Necrosis Factor
  • TNFRSF13B protein, human
  • Transcription Factor AP-1
  • Transcription Factors
  • Transmembrane Activator and CAML Interactor Protein
  • Calcineurin
  • Phosphoprotein Phosphatases

Associated data

  • GENBANK/AF023614