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Biochem Biophys Res Commun. 1997 Sep 8;238(1):113-8.

Primer/template-independent synthesis of poly d(A-T) by Taq polymerase.

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  • 1Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.


Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not. The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers.

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