Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Acta Otolaryngol. 1997 Jul;117(4):628-33.

A simple and reliable technique for culturing of human oral keratinocytes and fibroblasts.

Author information

  • 1Department of Otolaryngology/Head and Neck Surgery, University Hospital Vrigje Universiteit, Amsterdam, The Netherlands.

Abstract

Cultures of non-malignant oral cells are needed for many applications in the field of oral biology. We describe an efficient and rapid method for the culture of both keratinocytes and fibroblasts isolated from oral tissue, uvulas from patients undergoing reconstructive surgery and non-cancerous tissue from oral cancer patients. High cell yields with a high purity could be obtained. The technique is described in detail with respect to isolation of the cell types, the best way to propagate for over 5 passages and to measure cell proliferation in a colorimetric assay. Culture was successful in over 80% of cases for keratinocytes and over 63% for fibroblasts. Problems encountered were the risk of microbiological infection, the size of the specimen and the type of culture medium used. The potential applications of these primary cell cultures are mentioned.

PMID:
9288225
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk