Chem Res Toxicol. 1997 Aug;10(8):906-14.

Binding of nickel(II) and copper(II) to the N-terminal sequence of human protamine HP2.

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Laboratory of Comparative Carcinogenesis, National Cancer Institute, FCRDC, Frederick, Maryland 21702, USA.

Abstract

A potentiometric and spectroscopic (UV/vis and CD) study of Cu(II) and Ni(II) binding to the N-terminal pentadecapeptide of human protamine HP2 (HP2(1-15)) was performed. The results indicate that the N-terminal tripeptide motif Arg-Thr-His is the exclusive binding site for both metal ions at a metal to HP2(1-15) molar ratio not higher than 1. The very high value of protonation-corrected stability constant (log *K) for Ni(II)-HP2(1-15) complex, -19.29, indicates that HP2 has the potential to sequester Ni(II) from other peptide and protein carriers, including albumin. The same is likely for Cu(II) (log *K = -13.13). The CD spectra of Cu(II) and Ni(II) complexes of HP2(1-15) indicate that the N-terminal metal binding affects the overall conformation of the peptide that, in turn, may alter interaction of HP2 with DNA. These results imply HP2 as a likely target for the toxic metals Ni(II) and Cu(II).

PMID:: 9282840; [PubMed - indexed for MEDLINE]

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Mediation of oxidative DNA damage by nickel(II) and copper(II) complexes with the N-terminal sequence of human protamine HP2. [Chem Res Toxicol. 1997]
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Cited by 2 PubMed Central articles

Oxidative stress level in the testes of mice and rats during nickel intoxication. [ScientificWorldJournal. 2012]
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Murawska-Ciałowicz E, Bal W, Januszewska L, Zawadzki M, Rychel J, Zuwała-Jagiełło J. ScientificWorldJournal. 2012; 2012:395741. Epub 2012 Feb 1.
Diastereoselective DNA cleavage recognition by Ni(II) x Gly-Gly-His-derived metallopeptides. [J Am Chem Soc. 2006]
Diastereoselective DNA cleavage recognition by Ni(II) x Gly-Gly-His-derived metallopeptides.
Fang YY, Claussen CA, Lipkowitz KB, Long EC. J Am Chem Soc. 2006 Mar 15; 128(10):3198-207.

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