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J Chromatogr B Biomed Sci Appl. 1997 Jul 18;695(1):113-5.

High-speed electrophoretic separation of DNA fragments using a short capillary.

Author information

  • 1Chemical Synthesis and Analysis Laboratory, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702, USA.

Abstract

Capillary electrophoresis using a replaceable gel buffer was applied to the separation of DNA fragments. A short effective length capillary (1-2 cm) at low electric field allowed the separation of a 20-1000 bp ladder in 1 min. Although similar separation speed was achieved with a longer capillary at high field, the resolution of larger fragments was degraded. The short effective length capillaries were able to separate the wildtype and mutant PCR products of the TGF-beta1 gene in under 45 s.

PMID:
9271135
[PubMed - indexed for MEDLINE]
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