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Curr Opin Struct Biol. 1997 Aug;7(4):537-42.

Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.

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  • 1Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024-1662, USA. RonaldK@HHMI.UCLA.edu

Abstract

Recent advances in protein engineering have facilitated the development of alternative approaches to determine helix packing in polytopic membrane proteins. Using the lac permease as a paradigm, several site-directed biophysical and biochemical techniques are described which should be generally applicable.

PMID:
9266176
[PubMed - indexed for MEDLINE]
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