This article describes a study where the trace elements (TEs) of four groups of volunteers were analyzed. The volunteers were divided into four groups A, B, C, and D. Group A was made up of healthy subjects, group B was made up of volunteers who had just been diagnosed as having naso-pharyngeal cancer (NPC), group C was made up of volunteers who had been diagnosed as having NPC after 3 mo of treatment, and group D was made up of volunteers who had been diagnosed as having NPC after 6 mo of treatment. In all groups, 11 trace elements in hair were analyzed. Our study shows that the concentrations of zinc (Zn), copper (Cu), manganese (Mn), and cobalt (Co) in group B are less than that of group A, whereas the concentration of titanium (Ti) in group B is higher. Our results also show that the difference in the level of TEs between group A and the other groups is dramatically decreased as the time of the treatment is increased. This may be a reflection of successful treatment.